Insertion junction: CLIP2.012315_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.012315
Chromosome:chromosome 16
Location:4677736
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g667350 CAV6 (1 of 2) PF00520//PF08016//PF16905 - Ion transport protein (Ion_trans) // Polycystin cation channel (PKD_channel) // Voltage-dependent L-type calcium channel, IQ-associated (GPHH); Voltage-gated Ca2+ channel, alpha subunit intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTAATTAAGAAGTGAAGTCGTAGGGGGGGGGGTTGCAGGGATGTTTGG
Internal bar code:GGTTCGAGGGTCTCTTTTTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:65
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AAGAAACGCACAACACGTCG
Suggested primer 2:CTTCACGCTCCTGAACCTGT