Insertion junction: CLIP2.012326_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.012326
Chromosome:chromosome 3
Location:93191
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g800279 (1 of 1) PF00059//PF13229 - Lectin C-type domain (Lectin_C) // Right handed beta helix region (Beta_helix) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGCTGAGTCCCAGGGCTGGACGGCTAGTGCGCTTGGGGTGCCGTGTT
Internal bar code:CTTCCGCGACGCATGAGCCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:4543
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GGACTCCTTGTAAGGTGGGC
Suggested primer 2:GCACGAATAACATCACCGCC