Insertion junction: CLIP2.012329_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.012329
Chromosome:chromosome 6
Location:3448370
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278103 (1 of 4) 1.14.17.1 - Dopamine beta-monooxygenase / Dopamine beta-hydroxylase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGATCCCTTACCGCCTTGACCACCACCCCCACCCCCAACCCAACCCCAC
Internal bar code:TTAACTTCGGGATGAAAATCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:493
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACACGTAAACAGCACGC
Suggested primer 2:GGTCCACAAGAAGAAGCCGA