Insertion junction: CLIP2.012366_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.012366
Chromosome:chromosome 3
Location:6579049
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g194600 (1 of 2) IPR000719//IPR011009//IPR013221 - Protein kinase domain // Protein kinase-like domain // Mur ligase, central intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTAACTCAGCCTCGCGCGGACGTTGGACGAGCGAGTCGCGCTGGTTCG
Internal bar code:AATAGATAGACGTCTTTTCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3655
LEAP-Seq percent confirming:20.5882
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGATCCACCTCCATCTGG
Suggested primer 2:CGGCCAATTGTCCGCAATAG