Insertion junction: CLIP2.012377_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.012377
Chromosome:chromosome 12
Location:510823
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g494252 (1 of 1) PF14822 - Vasohibin (Vasohibin) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACTGCCCCGCTGCCCGGCGGCAGCCCCGCATCCCCCACCCGCAACGCA
Internal bar code:GGTGACCCTGCTAGACACATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:3133
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCTCCAGGGTCTGATGA
Suggested primer 2:AGGCAGGGAGAGAAGGAGAG