Insertion junction: CLIP2.012386_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.012386
Chromosome:chromosome 16
Location:836811
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g801885 (1 of 1) K15264 - putative methyltransferase [EC:2.1.1.-] (NSUN5, WBSCR20) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCCTCCACCCCACCTGTACACAAGCCCCTCCCTCCACCCCACCTGTAC
Internal bar code:TGCGGTTCGTCATTTGACTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:55
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTTTGTGTTGGGCAGGTC
Suggested primer 2:TCCACCAACACCAACACCAA