Insertion junction: CLIP2.012403_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.012403
Chromosome:chromosome 14
Location:1199324
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g615650 (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGGACGCCCCTCCCGTCATACCGAACATGTTACTAGGTGCCATCACTCC
Internal bar code:CTTAGGTTTAACATTATCAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:494
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGTATGAACACGGCGGGAAA
Suggested primer 2:ATCAACAATGCCCTGACGGT