Insertion junction: CLIP2.012410_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.012410
Chromosome:chromosome 4
Location:2876365
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g224400 (1 of 1) 3.6.3.25//3.6.3.44 - Sulfate-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGCGATGCCGTTCTGGACGCAGACGCGCGTGCTGCTGCGCAAGTACAA
Internal bar code:AAAGTAGAAGTTAGAAACATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2401
LEAP-Seq percent confirming:95.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAGGTGGAAAAGGCAGGC
Suggested primer 2:GTGATGTGAGGTCTGTGCCA