Insertion junction: CLIP2.012419_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.012419
Chromosome:chromosome 4
Location:2800245
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g223900 (1 of 2) K09313 - homeobox protein cut-like (CUTL) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTGGCTGAGGCTCCGACTGTGCCTGTGTGTAGGTGGAGGCCACGCTGC
Internal bar code:TTGTTGGACACTTTTGGTCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1437
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACCT
Suggested primer 2:GCTGCATAAGCGAGTCGTTG