| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.012421 |
| Chromosome: | chromosome 14 |
| Location: | 526157 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611400 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCACCCGCGGCGGCCTGCGCTCGCGCGGCGGGCACTGCCTGCACGCCG |
| Internal bar code: | TCAGCCTCAAGCGCCTCTAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2038 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAGCTCCAATGCAACAA |
| Suggested primer 2: | TGGACAGCATGAAGAGCCTG |