| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.012425 |
| Chromosome: | chromosome 14 |
| Location: | 3112349 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628400 | TSA3 | (1 of 3) 6.1.1.7 - Alanine--tRNA ligase / Alanyl-tRNA synthetase; Alanyl-tRNA-synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTCTAATTGCGTCAGGGACCGGCGAGACGGCGCGAAGGCTCGGAAGC |
| Internal bar code: | AACTGCTGGAGGTTCGTCGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5127 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGAACGTCAACTCGACA |
| Suggested primer 2: | TGCGTGTTTGGAAGCACATG |