Insertion junction: CLIP2.012451_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.012451
Chromosome:chromosome 12
Location:4858796
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g523650 HLM19 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase; Histone-lysine N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAGTCCAACCTGCCCTACACCCCTGACTTGCGCCGCGCCGCACCTGGC
Internal bar code:GTCGCGGTATCGTTCTTTTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1925
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GGATCCAGTTTCACTCGGCA
Suggested primer 2:TTCCAGCTACCAACACCGAC