| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.012453 |
| Chromosome: | chromosome 4 |
| Location: | 3416998 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g227600 | (1 of 1) PTHR27000:SF77 - LEUCINE-RICH REPEAT PROTEIN KINASE-LIKE PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCGCCGACCCAAGGGCCCGTGGGTTTGCTAAACCCGATGAGTTCTG |
| Internal bar code: | GGACGGTCGTTCGAGTCCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAGGGATGGTGCCTTGCA |
| Suggested primer 2: | CTACAACAGGGTGAGGCAGG |