Insertion junction: CLIP2.012454_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.012454
Chromosome:chromosome 9
Location:3321524
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g395362 (1 of 4) 1.13.11.75 - All-trans-8'-apo-beta-carotenal 15,15'-oxygenase / 8'-apo-beta-carotenal 15,15'-oxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGCGCCGCCGGGGGGCGGCAGTAGCGGGTGGGTGTGGCGCAGCGAGA
Internal bar code:TCGATACCAGGGTACTGCTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1072
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACACCCTACACACTCAC
Suggested primer 2:GTCTTTCCCTCCCTTCCACG