| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.012463 |
| Chromosome: | chromosome 16 |
| Location: | 5121199 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685450 | DHC8 | Axonemal Dynein Heavy Chain 8; (1 of 3) PTHR10676//PTHR10676:SF242 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // DYNEIN HEAVY CHAIN 3, AXONEMAL | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGCCGGCGCTTGGACGCTGGGTGCAGCGGGGCATGCGGTGGCAGGG |
| Internal bar code: | ACTGCGACGGAAAGAGCAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1181 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTAGTTGCGGAAGAAGCC |
| Suggested primer 2: | ATCGCGCGAGTCACTTCTAG |