Insertion junction: CLIP2.012481_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.012481
Chromosome:chromosome 1
Location:2330063
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g013050 (1 of 2) IPR006652//IPR011043 - Kelch repeat type 1 // Galactose oxidase/kelch, beta-propeller intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAACTACCAACCCCCTACACCACGCAGGCGGGCGGCTATGCTGCTGAGA
Internal bar code:CACTGGTCAAGGCCCGGTGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:62
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGGTGAGACGGAGAGAGT
Suggested primer 2:GACCTCATTGCCATTCGTGC