| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.012496 |
| Chromosome: | chromosome 14 |
| Location: | 175293 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g608750 | intron | ||
| Cre14.g801596 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTCAACGTCAGACTACTCACCGTCAGGCGGGCGGGCGGGCAAGAGCCT |
| Internal bar code: | TAGAGAGCGGACACATCGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1353 |
| LEAP-Seq percent confirming: | 69.697 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGGTGGAAACAAGGGCG |
| Suggested primer 2: | CGTTCCCTCTCTTTTCCCCC |