| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.012498 |
| Chromosome: | chromosome 4 |
| Location: | 3092576 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225500 | CSA6 | (1 of 1) PF15066 - Cancer-associated gene protein 1 family (CAGE1); conserved protein of unknown function | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACTAAAGGAGCGCGGAGCGGCTTACCCTGTCCCAAAAAATACGATGA |
| Internal bar code: | CGCGTACTTTCTATGACTGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3160 |
| LEAP-Seq percent confirming: | 97.0149 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGACAGTTGGTGCTAGT |
| Suggested primer 2: | GGTGAATGCCAGTAGGTGCT |