| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012508 |
| Chromosome: | chromosome 3 |
| Location: | 3149983 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164850 | (1 of 5) PF13391 - HNH endonuclease (HNH_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGACCCCCATGCTCCTCGCCTTCGGAGAGGGGAGGAACCCCAGGCCCG |
| Internal bar code: | GGACCGTAGGATCAGTGATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2448 |
| LEAP-Seq percent confirming: | 90.3226 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGACATCACGTGGAACCAG |
| Suggested primer 2: | TCCATCCTACCCTCCACCTG |