| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.012509 |
| Chromosome: | chromosome 12 |
| Location: | 521612 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801346 | (1 of 10) PF00176//PF00271 - SNF2 family N-terminal domain (SNF2_N) // Helicase conserved C-terminal domain (Helicase_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGCAACCCCCCCGACGACTACTGTGTACCTTTGTACCTGGCTACGAC |
| Internal bar code: | GTCAACGACGAGTGATGAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGACGTGTAGGGAGTATG |
| Suggested primer 2: | GGAGGAGGGGTAGAGTTCGT |