| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.012514 |
| Chromosome: | chromosome 13 |
| Location: | 1606786 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCAGCCGCGAACCAAGGGCGAAGGTTGTGCCGTGGCTTCATACCGG |
| Internal bar code: | CTAAGTCATTCGAGCTATACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2060 |
| LEAP-Seq percent confirming: | 91.8919 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCCTCGCCCATATCTTG |
| Suggested primer 2: | GAGCGAGGTGGAGGATTAGC |