| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.012526 |
| Chromosome: | chromosome 12 |
| Location: | 5357070 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528950 | PUF1 | Puf protein; (1 of 1) K14844 - pumilio homology domain family member 6 (PUF6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTAGTCCCAACAGTCTGAGGGGGAACTTTGGTCGGTACCCCCCTTCTT |
| Internal bar code: | GGGAGTTACACAACAGTGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2008 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGTGACCTTGGTAAGAA |
| Suggested primer 2: | GCAATGCCATGTGCTCTGAG |