Insertion junction: CLIP2.012585_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.012585
Chromosome:chromosome 2
Location:5461113
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g114150 outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATCTTAAGCTATACACGCGAACCGACATGCATAGGCTCAAACACCAGA
Internal bar code:GGGGTTCAGGATCGAGAGGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1842
LEAP-Seq percent confirming:65.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGTTTATGCCACCGTCG
Suggested primer 2:GTGCAAAATCCACCGTGCTT