| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.012586 |
| Chromosome: | chromosome 2 |
| Location: | 4830670 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109200 | OPR123 | OctotricoPeptide Repeat protein 123 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGCGGCCTTTGACCAGCGCGTGGCGGCGGCGGAGATGGCGCCACACG |
| Internal bar code: | GAAGATTTTACAGTCAGGAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1625 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCGCCTTCAATAACATG |
| Suggested primer 2: | CATGTCCTGCGCTAACCTCA |