Insertion junction: CLIP2.012594_4

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.012594
Chromosome:chromosome 10
Location:1460444
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g428300 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATTGATCACGGCAAGTCGCACCAATTCACCGGCGCCATGTCAGCTTTT
Internal bar code:AGGGATAATGTTAATGCCATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2325
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACCCTGCTGATCAACTG
Suggested primer 2:GTCCCATGCCTACAACAGCT