Insertion junction: CLIP2.012613_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.012613
Chromosome:chromosome 15
Location:2149732
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g142366 (1 of 4) IPR003882//IPR008752 - Pistil-specific extensin-like protein // Peptidase M11, gametolysin CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGACATGAACAACAAGGTGTGTGTGTGGAGGGTGTGGTGGAGAGGATAG
Internal bar code:CTTCTAAGTTTTCGATTTCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1039
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAAATGACGGGGCTTCGT
Suggested primer 2:CATTGTGCCCCCTCCTTCAT