Insertion junction: CLIP2.012615_4

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.012615
Chromosome:chromosome 13
Location:3850385
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGACCGCGAGGTCGTGACCAAGGTCAAGCCCCGCGCCGCGGCTCGTG
Internal bar code:TTGGAAGGTATTTACTATATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1205
LEAP-Seq percent confirming:9.09091
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGAGGACGTAGGTCACCT
Suggested primer 2:CTACATGCAAACGAGCGCTC