| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.012622 |
| Chromosome: | chromosome 13 |
| Location: | 2611679 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580900 | (1 of 1) K07178 - RIO kinase 1 (RIOK1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAAAAAGAAGTAGAGCGGTGTCGAGGGTCGTGCGTGTGGTGGACATGA |
| Internal bar code: | CCCATCAAGCCTAAATTGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4075 |
| LEAP-Seq percent confirming: | 98.9796 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCGCTGTGATTTTGGAAA |
| Suggested primer 2: | ATCCAAGTCCAAGAAGGCGG |