| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.012624 |
| Chromosome: | chromosome 6 |
| Location: | 3908362 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278190 | (1 of 5) PTHR10351:SF42 - LD40663P | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTTTGAGTTGGTTAGGCTTGGGCTGAGTCATGAGTGGGGGCCTGGTTC |
| Internal bar code: | GAATGGGGTCTCAATCGGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1830 |
| LEAP-Seq percent confirming: | 79.1667 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACACGAGTGCCTGACTGA |
| Suggested primer 2: | CCCTTGATCACGTGTGGGAA |