| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.012661 |
| Chromosome: | chromosome 10 |
| Location: | 3876222 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446275 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTAATGTCCAACAATGCCGGGACCGACGCAGCGCCCACCGCCGTCACCT |
| Internal bar code: | TGGAGACTTAGCTGCTACGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3293 |
| LEAP-Seq percent confirming: | 80.5755 |
| LEAP-Seq n confirming: | 112 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 139 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGACCTCCACACACGAG |
| Suggested primer 2: | ATACAACCGCTTCACCTCCG |