Insertion junction: CLIP2.012686_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.012686
Chromosome:chromosome 16
Location:1695595
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g654700 PHC61 Pherophorin-chlamydomonas homolog 61; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGCCGGCAGGGGCGGGGCTGCTGCCAGTGCCAGCAGGCGAAGGGCTGC
Internal bar code:GATAATACGATGGTGACGCAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3609
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCCTTCTACTGGTGGTG
Suggested primer 2:GCTGCTCCCAGTGTCAGTAG