| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.012695 |
| Chromosome: | chromosome 12 |
| Location: | 8386874 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550400 | GRX2 | Glutaredoxin, CPYC type, cytosolic; (1 of 2) K03676 - glutaredoxin 3 (grxC, GLRX, GLRX2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCCAAACAGCAAAGAGTCACAAGTATAAGGCGAGTTGTTTCAGCGTT |
| Internal bar code: | TTATAAGTCGATGTTGATATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1920 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCTGGGCTTTCATCTTC |
| Suggested primer 2: | GCAGTAGGGGCAGTACGTTT |