| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012697 |
| Chromosome: | chromosome 5 |
| Location: | 2771150 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240850 | THIC1,THIC,THI8 | (1 of 1) 4.1.99.17 - Phosphomethylpyrimidine synthase; Hydroxymethylpyrimidine phosphate synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGACTGACAAGTTTGGGAAGTACGGCTGCCGCTTGGCAGTAGCAGC |
| Internal bar code: | TCATAACTTGATTAATAGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2040 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCAGTTGTGAGGGTA |
| Suggested primer 2: | GAGGATGGGCCGGAAAGAAA |