| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.012705 |
| Chromosome: | chromosome 13 |
| Location: | 2986716 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583972 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCACCACAGCAAGCCAGGCGTGATGCAGCTGCACGACCACAGTTCACT |
| Internal bar code: | GAGGCGACACCTTCTGTACCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2949 |
| LEAP-Seq percent confirming: | 79.5455 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGAACCTTTGCCAGTTG |
| Suggested primer 2: | CAGCCAAACAAGAAGCCACC |