| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.012716 |
| Chromosome: | chromosome 16 |
| Location: | 3599688 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671685 | (1 of 5) 2.3.1.24 - Sphingosine N-acyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTAAACTAGGGAATAGAAGAGGTAGCGCAATGCCAGGACTGCAGCGC |
| Internal bar code: | TTTCTGTAGATTCGTTTGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3177 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTGGCTGTTGCATTTGC |
| Suggested primer 2: | CCACACCAGTAAGAGGCCAG |