| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012724 |
| Chromosome: | chromosome 17 |
| Location: | 2663479 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716850 | (1 of 1) PTHR23327//PTHR23327:SF9 - RING FINGER PROTEIN 127 // ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN | intron | |
| Cre17.g716900 | (1 of 1) IPR002104//IPR010998//IPR011010 - Integrase, catalytic domain // Integrase, Lambda-type, N-terminal // DNA breaking-rejoining enzyme, catalytic core | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGGCCATCGCGGCCGGCAGCTCTACTCACTCGTCAGCCCCAGCAGCA |
| Internal bar code: | GAGTGACATAGGGACGTTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3208 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGTTGGCTGGTCTGGTT |
| Suggested primer 2: | AACATATTCGAGCCGCGCTA |