Insertion junction: CLIP2.012729_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.012729
Chromosome:chromosome 6
Location:6646122
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g294850 UBC10 Ubiquitin-conjugating enzyme E2; (1 of 1) PTHR24067:SF142 - UBIQUITIN-CONJUGATING ENZYME E2 3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGACGAAGCTGTTCCACCCCAACGGTGAGCACGCGCCTCCCCCAACGC
Internal bar code:GGTATTGACCGGCGATAGCCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3992
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:CCACGCTTTCAACACATGCA
Suggested primer 2:CTGAAATCCGCCAGCGATTG