| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.012802 |
| Chromosome: | chromosome 7 |
| Location: | 1161294 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321000 | CYG14 | (1 of 1) PF00211//PF01590 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // GAF domain (GAF); Adenylate/guanylate cyclase | CDS |
| Cre07.g321050 | (1 of 1) PTHR11772//PTHR11772:SF3 - ASPARAGINE SYNTHETASE // ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTGCCAGCGCGTTGATGGCTCGGCCGTGGCGGTGGTACAGGCCCCCG |
| Internal bar code: | AGGTGGATTCACCGGGGTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1605 |
| LEAP-Seq percent confirming: | 86.3636 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACCACGAGTGCAAGGTG |
| Suggested primer 2: | ACCGTGTCAGAAGGAAGCAG |