| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012805 |
| Chromosome: | chromosome 12 |
| Location: | 8199833 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551801 | (1 of 1) PTHR12100//PTHR12100:SF0 - SEC10 // EXOCYST COMPLEX COMPONENT 5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACACACACACACACACACTCCCCACGCATACACTGTCTTTGCGCTAT |
| Internal bar code: | GTCTTGAGAAAAGTTTACCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGCACTTCGGTCTTGCA |
| Suggested primer 2: | CCCTCCGCTAACCTTGTTGT |