| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.012828 |
| Chromosome: | chromosome 10 |
| Location: | 935096 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424350 | (1 of 1) PTHR24209:SF7 - PROTEIN DA1-RELATED 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAATGGCACCCGGACTGTTTCAAGTGAGTGTCGGAGTGGCGGGGTTGT |
| Internal bar code: | ACCGGCTTTGCGTGGTGTGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTAGCAGTCGGTGTGGT |
| Suggested primer 2: | GCTACTATCCCCATGTGCCC |