| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.012835 |
| Chromosome: | chromosome 1 |
| Location: | 7754775 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054600 | (1 of 8) IPR000157 - Toll/interleukin-1 receptor homology (TIR) domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCAGGACAGGAGGCGGGTGGTGGGTGGCGCGGCAGCAGTGCACCGCA |
| Internal bar code: | ATATACCACGTTAGCATGTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1206 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCTGGAGTGGAAGGAAT |
| Suggested primer 2: | AGGGTTACCGGTCGTACGTA |