| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012867 |
| Chromosome: | chromosome 16 |
| Location: | 1786507 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g655100 | (1 of 1) PF04266 - ASCH domain (ASCH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGGCACATGCACGGGTTGTGGGTGTTGGAGTACGAACGGTACCCTAG |
| Internal bar code: | GACAATGCTCACAACGAAAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2929 |
| LEAP-Seq percent confirming: | 93.4211 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCACACAAGCCAGCTAGC |
| Suggested primer 2: | TGCGTGAGATGCCAGACATT |