| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012870 |
| Chromosome: | chromosome 10 |
| Location: | 763494 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422750 | MOT10 | Predicted protein; (1 of 3) IPR002110//IPR005821//IPR020683 - Ankyrin repeat // Ion transport domain // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCCGTTGCCCTCGTACAGCTGGTAGTCGCCATTGGCCAGTGTGATCT |
| Internal bar code: | TATGCTCTGAACAATAGCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3146 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCATGAGCCTTGACCATT |
| Suggested primer 2: | CTAATCAGGTGGCGGTGGTT |