Insertion junction: CLIP2.012888_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.012888
Chromosome:chromosome 10
Location:2191418
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g801121 (1 of 1) PTHR10459:SF7 - DNA LIGASE 4 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCAGCGGTATGCCGGCCTCCGGTGCGTCACGCAGCGCCTTGCGGACCA
Internal bar code:TGAAACAGTATGTAGTAGTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:662
LEAP-Seq percent confirming:76.9231
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ATGACGTCAGACCCCGACTA
Suggested primer 2:CTGGGTAGGCCAATGTGTGT