| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012912 |
| Chromosome: | chromosome 10 |
| Location: | 2023006 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432400 | OPR46 | OctotricoPeptide Repeat protein 46 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGTGAGCGAGCAGCGGCATAGTAGTGCAAGTGCTTGGAATGATGAGC |
| Internal bar code: | AGAATAGCAAACGTGGACTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2182 |
| LEAP-Seq percent confirming: | 88.2353 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTAGCCGCTCATCGTACA |
| Suggested primer 2: | CATGCTCGCGAAGAACCATG |