| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.012914 |
| Chromosome: | chromosome 17 |
| Location: | 5211231 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g737100 | FAP127 | (1 of 1) PTHR19265:SF0 - MEIOSIS-SPECIFIC NUCLEAR STRUCTURAL PROTEIN 1; Flagellar Associated Protein 127 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGGAGATGGAGGCGGAGATGGCCAGGTGAGCTATGGCCCCAAATGCA |
| Internal bar code: | GTGAGTGTAGGTGGTGTGTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2641 |
| LEAP-Seq percent confirming: | 99.2754 |
| LEAP-Seq n confirming: | 137 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 138 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCGGGTAGTGTTGTGTA |
| Suggested primer 2: | CCACATGCCCTCCACTTCAT |