Insertion junction: CLIP2.012919_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.012919
Chromosome:chromosome 12
Location:6183483
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g535800 (1 of 1) PF13191//PF13424 - AAA ATPase domain (AAA_16) // Tetratricopeptide repeat (TPR_12) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCCCCACGCACAGAACAAGCTGCCGGAGGCGGAGGCGGCGTGTCGGGA
Internal bar code:GCAACTTTAGGCGCTTCCGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1288
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGCCTTCGCTTTCTGGAT
Suggested primer 2:CAAAGTCCCATCACCCGTCA