Insertion junction: CLIP2.012920_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.012920
Chromosome:chromosome 14
Location:2650961
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g625500 ANK10,FAP254 Ankyrin Repeat Flagellar Associated Protein 254; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGTCCAGATATAACCTGGACAAGTACAACCTGTTCGCGCCCAGCTGAG
Internal bar code:CTTGTGCTTCTGCAAGCGTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2218
LEAP-Seq percent confirming:91.8367
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCGGATTACAATTGCGGC
Suggested primer 2:CGTAATGCCGTGGCTCATTG