Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | - |
Strain: | CLIP2.012923 |
Chromosome: | chromosome 6 |
Location: | 3234107 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g276900 | HTB24,HTB1 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAGGCTGAGGCCAAGCCCAAGGCCGAGAAGGCCGCCAAGAAGGCCAA |
Internal bar code: | GTAGCGGTGTGTTAGCCGTTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1615 |
LEAP-Seq percent confirming: | 21.4286 |
LEAP-Seq n confirming: | 12 |
LEAP-Seq n nonconfirming: | 44 |
LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCTCAAATCCCCAAGTCCC |
Suggested primer 2: | CGAGTTGGGGGTTTTTGCAG |