Insertion junction: CLIP2.012931_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.012931
Chromosome:chromosome 12
Location:1992114
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g510950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACAAGGCCGCAATCACGCACTCGACCATTCCGGGCGCGTACCATCAA
Internal bar code:GGCTGCTATAACAGGTGCACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:663
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCATTAAAGCTGGTGCC
Suggested primer 2:CACTGTCAGGTTGTGCAACG